To complement the applied approaches we are able to take in the IPL, we have numerous capabilities as well in our collective wet lab space. These labs are located in the Applied Health Sciences building (AHSB) on the 1st and 6th floors. We are able to perform a variety of biotechnology and physiological analyses that include protein (Western blots, ELISAs, multiplex assays), gene expression (Qt-RT PCR), cell culture and studies on isolated microvessel function. On the first floor, over 10,000 ft2 is devoted to the faculty, staff and students and this space is equipped for biochemical, molecular, cellular, and vascular physiology. Essential equipment includes a Biorad Protean Mini Western Blotting System, Luminex Magpix multiplexing platform, Molecular Devices Spectra Max plate reader, and a Kodak 400R Imaging Station for Western Blot development and quantification. In addition, cell culture, flow cytometry and fluorescent microscopy are part of the general shared laboratory space.
The VHL, directed by Dr. Michael Brown has over 645 ft2 of area on the 6th floor, which is dedicated to cell culture models and equipped for biochemical, molecular, cellular, and vascular physiology experiments. This includes a cell culture hood, cell incubators, cell counter, and other general lab equipment essential for execution of cell culture experiments. Other essential equipment, not previously mentioned, includes a cone and plate viscometer, inverted microscope with digital camera and image analyzing software, and a FluroChem imaging system for Western Blot development and quantification.
Vascular Biology Laboratory
The laboratory is directed by Dr. Shane Phillips is dedicated to experimentation on isolated blood vessel function. This includes 4 complete modules for microvascular diameter measurements, fluorescent microscopy for the study of redox probes in blood vessels, and arterial tension measurements.
The NIH funded Clinical Research Center (CRC), supported by the Center for Clinical and Translational Services (CCTS) to UIC (UL1RR029879). The nurses of the CRC have experience with hyperinsulinemic-euglycemic clamps, skeletal muscle and adipose tissue biopsies and other metabolic testing. In addition, blood processing equipment and laboratory analysis is available in the CRC. The IPL has metabolic testing equipment housed in the CRC including: Parvo Medics True One resting metabolic rate unit with canopy, (2) YSI 2300 Stat Plus glucose and lactate analyzers, (6) calibrated Harvard PhD infusion pumps with adapters for multiple 140cc syringes and microdialysis preparations, heated hand box and multiple Bergstrom muscle biopsy needles.
The UIC CCTS has numerous translational CORE services available to CRC investigators.